Inactivator solutions are used in environmental monitoring microbiology to neutralise the residual activity of disinfectants. This is crucial to avoid false negative results. Many different disinfectants are being used to prevent biocontamination at manufacturing facilities, therefore it is important to select the appropriate neutraliser formulation.


Primary Use

Neutralising antimicrobial agents on surfaces or equipment, ensuring that any microorganisms present can grow without being inhibited by the disinfectants. Used as a wetting agent for swabs that are used for sampling inaccessible areas for contact plates, strips, or slides (e.g., corners, crevices, and equipment in the work zone).

A diluent for the neutralising of a range of disinfectants, as per ‘inactivation liquid’ in BS EN 1650: 2019 (European Suspension test).

The EN 1650 test method '"Chemical disinfectants and antiseptics - quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas" is a European standard that specifies the use of an appropriate neutralising solution during the testing process. The standard outlines that after the contact time of the disinfectant with the test organisms, the mixture must be neutralised using an appropriate neutralising solution to stop the action of the disinfectant and allow for accurate enumeration of surviving microorganisms.

Common Neutralising Agents:


Neutralising Agent Inactivates
Polysorbate 80 Phenolic compounds, hexachlorophene
Lecithin Quaternary ammonium compounds
Sodium Thiosulphate Iodine, chlorine-based disinfectants
Histidine Aldehydes, peroxides

Typical Composition:


Component Amount (per litre)
Lecithin 20.0 g
Tween 80 30.0 mL
Sodium Thiosulphate 10.0 g
Sodium Hydroxide 1.0 mL

pH - 7.0 ± 0.5

 

Does Plate Choice Change Sampling Accuracy?

Comparative Analysis of Petri Dish Variation in Flow Rate for Air Sampling. This extended report aims to further investigate the variations in flow rate observed in petri dishes used for air sampling, focusing on the potential factors contributing to these fluctuations.


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