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In addition to our standard range of Redipor® media products, we also offer triple-wrapped irradiated settle plates, 90 and 140mm Petri dishes and contact plates. Gamma irradiation extends shelf-life typically to 6 months in ambient conditions.
Finally, they are tested after irradiation to confirm growth properties and pH. A detailed certificate of analysis is provided with each batch.
Packaging plays an important role in maintaining conditions for the agar and providing consistent, reliable prepared media that delivers confidence in your sampling.
The Redipor triple-wrapped irradiated plates packaging illustration demonstrates the sterility assurance provided by each layer, following exposure to gamma radiation. It shows how the packaging allows the plates to be easily handled, while being transported through each stage of a facility, without the risk of introducing contaminants.
For use in environments where Vaporised Hydrogen peroxide (VHP) is deployed, such as Isolators, Redipor Barrier pack provides a validated, protective layer to prevent the ingress of VHP gas. Available for both 90mm Petri dishes and Contact plates, this product is supplied in standard sleeves of 10 plates.
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The sterility of the packaged medium is assured and all but the outer layer of packaging is also sterile. Thus the risk to the environment to be sampled is greatly reduced.
There is an additional benefit that the additional packaging and process extends the room temperature shelf life. This can be sufficient reason for small or irregular users to prefer irradiated.
Our Redipor Prepared Media range is so extensive that not every product is included in the official price list. In addition to offering such a wide selection of products, we have developed a flexible manufacturing process enabling us to produce bespoke solutions.
Contact us to discuss your requirements, whatever they are.
The majority of our prepared media can be stored in ambient conditions, not exceeding 25ºC. There are only a small number of very specialist products that require different storage conditions.
We have never specified refrigeration as a storage condition for our general media as this causes excess condensation and can result in very wet agar, rendering it impossible to use.
Settle plates are used to monitor the level of viable particles in the environment through a process of passive air sampling. A viable particle settles on agar plates at a rate dependent on its characteristics and the airflow in the environment.
EU GMP Guide Annex 1 has recommended that 90mm settle plates can be exposed in cleanroom environments for up to 4 hours. However, agar plates may dry out during long exposures where the rate of air exchange is high. So, it might be necessary to use deep filled settle plates, or replace the settle plate after a shorter time to ensure satisfactory growth promotion after exposure.
The final wrap of irradiated plates should be removed at the point of use and it is then a question of risk.
For example in a grade A isolator it might be reasonable to rewrap a part pack of plates for use in the next session. If all the exposed plates show no growth it is a reliable result.
However if one or more colonies appear on the plate it might not be possible to know during which session the contamination occurred and the OOT investigation becomes more complicated and expensive.
For Redipor media the Use By date is the last day on which the medium should be inoculated or exposed. Any incubation period that is normal for that product may begin on that day. For example a 14 day sterility test in TSB or a 5 day incubation for a TSA settle plate.
Not as obvious as it might sound. 3 layers of wrap between the agar plate and the outside world, of course, but Redipor can offer at least 3 different ways to achieve that with our standard product range.
We then have even more bespoke solutions to meet individual needs. Let us know how and where you want to remove the layers and we can find the best solution.