Other common names
Sabouraud Dextrose Agar (SDA), developed by Dr. Raymond Sabouraud in 1892, is a selective medium for fungal culture. It is primarily used to isolate dermatophytes, yeasts, and other fungi, both pathogenic and non-pathogenic. SDA is a complex medium for cultivation and isolation of yeasts and moulds as well as the absence test for Candida albicans.
According to pharmacopoeia and ISO 18415, the neutralisers lecithin, polysorbate (Tween®) 80, histidine and sodium thiosulfate are suitable for neutralisation of disinfectant residues containing the following active agents: aldehydes, bis-biguanides, oxidizing compounds, parabens, phenolic compounds, and quaternary ammonium compounds.
Commonly used in research and clinical studies, SDA traditionally inhibits bacterial growth through its low pH (5.6). The mycological peptone (a mixture of animal and plant peptones) and dextrose helps the rapid growth of the fungi.
The medium contains a variety of neutralising agents to reduce the growth inhibiting effects of a range of potentially interfering or antimicrobial substances.
The base formulation is prepared according to the recommendations of the current European, Japanese and United States Pharmacopoeia (EP, 2.6.12.; JP, 4.05 and USP, 61) and supplemented with neutralisers.
Primary Use
For the determination of the total count of yeasts and moulds on dry, sanitised surfaces and personnel in cleanrooms and isolators.
