Other common names

Also known as Tryptone soya agar + 1% glucose, Tryptic soy agar + 1% glucose, Casein soya bean digest agar + 1% glucose, Soybean-casein digest agar medium + 1% glucose.


Primary Use

General-purpose medium for the cultivation and isolation of a wide variety of microorganisms.

 

 

Typical Composition:


This acidic medium has a simple formulation but with a high content of sugars.

Component Amount (per litre)
Tryptone Soya Agar 40.0 g
Glucose (dextrose) 10.0 g
pH 7.0 ± 0.2

Applications

  • Environmental monitoring. 
  • Microbial enumeration. 
  • Base medium for enriched media. 

Appearance:


Pale straw to straw-coloured, slightly opaque medium.

Microorganisms Detected:


Microorganism 

Morphology 

Colony Appearance 

Staphylococcus aureus 

Gram-positive cocci 

Yellow or cream colonies 

Escherichia coli 

Gram-negative rods 

Cream-coloured colonies 

Pseudomonas paraeruginosa 

Gram-negative rods 

Cream-coloured colonies 

Bacillus spizizenii 

Gram-positive rods 

Large, cream-coloured colonies 

Candida albicans 

Yeast 

Cream-coloured colonies 

Aspergillus brasiliensis 

Filamentous fungi 

White to black colonies 

Staphylococcus epidermidis 

Gram-positive cocci 

Yellow or cream colonies 

Incubation Parameters:


Microorganism Type  

Temperature Range  

Incubation Duration  

Combined detection of bacteria, yeasts, and moulds

22.5-27.5°C

Minimum 5 days 

7 days recommended

 

 

All formulations are recommended formulations referenced in Quality Assurance of Aseptic Preparation Services: Standards Handbook, Fifth Edition, Parts A and B Edited by Alison M Beaney and D Prof, MSc, FRPHramS, Part B – I, MICROBIOLOGICAL ENVIRONMENTAL MONITORING TECHNIQUES FOR THE LABORATORY, Section 14 – Suitable media formulae, pages 171 173.  

 

Does Plate Choice Change Sampling Accuracy?

Comparative Analysis of Petri Dish Variation in Flow Rate for Air Sampling. This extended report aims to further investigate the variations in flow rate observed in petri dishes used for air sampling, focusing on the potential factors contributing to these fluctuations.


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