Growth Media Type Tryptic Soy Broth (TSB)

Other common names

TSB can be referred to using other names by the various manufacturers of dehydrated culture media, these include Casein Soya Bean Digest Broth, Soybean Casein Digest Broth, CASO Broth, Trypticase® Soy Broth and Tryptone Soya Broth. 

Primary Use

Tryptic Soy Broth is a general purpose highly nutritious liquid medium for the cultivation of a wide variety of fastidious and non-fastidious organisms, including both aerobes and anaerobes, although the latter may require anaerobic conditions. 

Technical characteristics

The medium’s simple formulation consists of two types of peptone and sodium chloride.

pH 7.3 ±0.2 

Applications

The main application for TSB is for performing microbial tests and for assessing the sterility of pharmaceuticals and medical devices, as well processes and operators. 

Tryptic Soy Broth can also be used at double strength to allow the addition of an equal miscible sample.

As with most liquid medium, growth of microorganisms shows as turbidity in the otherwise clear liquid. 

Related reading:

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