Other common names

This product is widely known under the XLD agar, although it can be referred to as xylose lysine deoxycholate agar.

 

Primary Use

A moderately selective medium recommended for isolation and differentiation of enteric pathogens, especially Shigella species.

Technical characteristics


The typical medium composition:

Typical formulation: g/ltr
Xylose 3.5
L-Lysine 5
Lactose 7.5
Saccharose 7.5
Sodium chloride 5
Yeast extract 3
Phenol red 0.08
Sodium Desoxycholate 2.5
Ferric ammonium citrate 0.8
Sodium Thiosulfate 6.8
Agar 15

Fresh pH 7.4 ±0.2

Applications

XLD agar is a selective and differential medium for the isolation of enteric pathogens such as Shigella or Salmonella species from food, and environmental samples.

It relies on xylose fermentation, lysine decarboxylation and production of hydrogen sulphide for the primary differentiation of shigellae and salmonellae from non-pathogenic bacteria.

Desoxycholate is a selective agent which inhibits the growth of Gram-positive microorganisms.

Appearance

The prepared media should appear as clear with red or pinkish-red colouration.


Organisms & morphology


Microorganism Observational appearance
Salmonella spp. Red with black center
Shigella Red colonies
Escherichia coli Yellow to yellow-red colonies

Download PDF version

Typical incubation parameters

XLD agar is incubated at 30 – 35 °C for 18 to 48 hours when culturing Salmonella as recommended in the Pharmacopoeia guidance for “Testing of products – Bile-Tolerant Gram Negative Bacteria – Salmonella”.


Regulatory references

USP<62> Microbiological Examination of Nonsterile Products: Test for Specified Microorganisms Table 1

Test for Salmonella

Test/Medium

Property

Test Strains

Xylose Lysine Deoxycholate Agar

Growth promoting + indicative

Salmonella enterica subsp. enterica serovar Typhimurium
or
Salmonella enterica subsp. enterica serovar Abony


Download PDF version

 

Does Plate Choice Change Sampling Accuracy?

Comparative Analysis of Petri Dish Variation in Flow Rate for Air Sampling. This extended report aims to further investigate the variations in flow rate observed in petri dishes used for air sampling, focusing on the potential factors contributing to these fluctuations.


Download Guide