This product is widely known under the XLD agar, although it can be referred to as xylose lysine deoxycholate agar.
A moderately selective medium recommended for isolation and differentiation of enteric pathogens, especially Shigella species.
The typical medium composition:
Typical formulation: | g/ltr |
Xylose | 3.5 |
L-Lysine | 5 |
Lactose | 7.5 |
Saccharose | 7.5 |
Sodium chloride | 5 |
Yeast extract | 3 |
Phenol red | 0.08 |
Sodium Desoxycholate | 2.5 |
Ferric ammonium citrate | 0.8 |
Sodium Thiosulfate | 6.8 |
Agar | 15 |
Fresh pH 7.4 ±0.2
XLD agar is a selective and differential medium for the isolation of enteric pathogens such as Shigella or Salmonella species from food, and environmental samples.
It relies on xylose fermentation, lysine decarboxylation and production of hydrogen sulphide for the primary differentiation of shigellae and salmonellae from non-pathogenic bacteria.
Desoxycholate is a selective agent which inhibits the growth of Gram-positive microorganisms.
The prepared media should appear as clear with red or pinkish-red colouration.
Microorganism | Observational appearance |
Salmonella spp. | Red with black center |
Shigella | Red colonies |
Escherichia coli | Yellow to yellow-red colonies |
XLD agar is incubated at 30 – 35 °C for 18 to 48 hours when culturing Salmonella as recommended in the Pharmacopoeia guidance for “Testing of products – Bile-Tolerant Gram Negative Bacteria – Salmonella”.
USP<62> Microbiological Examination of Nonsterile Products: Test for Specified Microorganisms Table 1
Test for Salmonella |
||
Test/Medium |
Property |
Test Strains |
Xylose Lysine Deoxycholate Agar |
Growth promoting + indicative |
Salmonella enterica subsp. enterica serovar Typhimurium |
Comparative Analysis of Petri Dish Variation in Flow Rate for Air Sampling. This extended report aims to further investigate the variations in flow rate observed in petri dishes used for air sampling, focusing on the potential factors contributing to these fluctuations.
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