By utilising the ISO-90 head and directly mounting it to a smaller ISO-CON system that incorporates low power electronics, the first battery powered, slit-to-agar air monitor, that is Annex 1 compliant, is now possible.
This self-contained monitor is available either as a battery powered unit, ImpactAir-90-B, with external PSU charger. Or as a mains only version, ImpactAir-90-M, with internal power supply.
Both version have a touch screen LCD user interface, which allows run parameters to be set up and can communicate with systems via Ethernet using MODBUS/SCADA.
4+ hours of continuous monitoring is possible using a single 9cm agar plate placed within the ISO-90 head. The head contains the controls needed to rotate the plate and control the air flow.
With a footprint of only 150mm x 200mm, this is an ideal option for general monitoring in pharmaceutical, healthcare and food production industries.
Exceptional biological efficiency
ImpactAir air monitors are based on the slit-to-agar method, which has very low d50 values of around 0.5µ with exceptional biological efficient and the ability to easily count real viable microbial events in CFU while being able to identify to species level.
Twin impingement probability reduced
Air is drawn at high speed through a narrow inlet. Particles and microbes are impacted on to agar in a rotating Petri dish. Fresh agar is presented constantly, significantly reducing the chance of multiple microbes appearing as a single colony after incubation.
Continuous monitoring using a 9cm agar plate
The rotating agar plate means results are time correlated. Whilst not timed to the second, localised growth on the plate can be time estimate based on its position on the plate.
Typical requirements suggest 1,000 litres per air sample in high risk areas, such as: grade A filling lines, grade B clean rooms, operating theatres etc. As the criticality of the area reduces, the sample size can be reduced. The aim is to achieve a representative sample; so where higher counts would be expected, a smaller sample produces a more realistic number of cfu to count.
SAS samplers were originally designed for Contact plates, however, a Petri dish option has been available for a number of years. It is really a personal choice, although this should be decided at time of purchase, as the sampler will be specifically configured for the plate type chosen. There are advantages for each version and we would be happy to discuss your specific needs.
No, do not put your SAS sampler in a steam autoclave. The only part that can be autoclaved is the drilled head. The unit can be wiped with alcohol wipes to decontaminate it. The only other exception is the SAS Pinocchio, parts of which can be autoclaved.
Cherwell Laboratories recommends every 12 months and we will send a reminder for the month it is due. For some situations local procedures demand more frequent recalibrations so Cherwell is happy to offer tailored recalibration date labelling and reminders on request.
The sterility of the packaged medium is assured and all but the outer layer of packaging is also sterile. Thus the risk to the environment to be sampled is greatly reduced. There is an additional benefit that the additional packaging and process extends the room temperature shelf life. This can be sufficient reason for small or irregular users to prefer irradiated.
Settle plates are used to monitor the level of viable particles in the environment through a process of passive air sampling. A viable particle settles on agar plates at a rate dependent on its characteristics and the airflow in the environment.
EU GMP Guide Annex 1 has recommended that 90mm settle plates can be exposed in cleanroom environments for up to 4 hours. However, agar plates may dry out during long exposures where the rate of air exchange is high. So, it might be necessary to use deep filled settle plates, or replace the settle plate after a shorter time to ensure satisfactory growth promotion after exposure.
The storage condition for the majority of our prepared media is Ambient not exceeding 25ºC, the exception being a couple of very specialist products.
We have never specified storage in a fridge for our general media as this causes excessive condensation and can result in a very wet agar surface. This makes the product impossible to use.
General purpose media have nutrients that support the growth of most non fastidious culturable microorganisms. Selective growth media contain components that will inhibit the growth of some types of microorganisms, while supporting the growth of others.
General purpose media, such as Tryptone Soya Agar, are used to produce total counts. While selective media, such as XLD for Salmonella species, are used to test presence/absence of specific types of microorganism.