Other Common Names

This product is widely known as Luria broth agar, although it can be referred to as Luria Bertani agar or Luria agar.

Primary Use

Media for the cultivation and maintenance of Escherichia coli.

 

 

Technical characteristics


The typical medium composition:

Typical formulation: g/ltr
Phytone peptone 10
Yeast extract 5
Sodium chloride 5
Agar 15

Fresh pH 7.0 ± 0.2

Applications

LB Agar is a medium used for general growth and maintenance of Escherichia coli strains. This medium is typically used in molecular biology procedures for cultivation of recombinant strains of E. coli for the detection of phage or plasmid transformed bacteria

Appearance

The prepared media is pale straw to straw in colour with a slightly opaque appearance.



Organisms & morphology


Microorganism Observational appearance
Escherichia coli Off-white colonies

Download PDF version

Typical incubation parameters

Incubation conditions can vary depending on the specific strain requirements. Typically, incubation range are within 35-37 °C for 18-24 hours.


Download PDF version

 

Related reading:

 

Does Plate Choice Change Sampling Accuracy?

Comparative Analysis of Petri Dish Variation in Flow Rate for Air Sampling. This extended report aims to further investigate the variations in flow rate observed in petri dishes used for air sampling, focusing on the potential factors contributing to these fluctuations.


Download Guide