Growth Media Type Luria Broth Agar

Luria broth (LB) agar contains the generic components such as peptone, yeast extract, sodium chloride (NaCl) and agar which is useful to support microbial growth

Other Common Names

This product is widely known as Luria broth agar, although it can be referred to as Luria Bertani agar or Luria agar.

Primary Use

Media for the cultivation and maintenance of Escherichia coli.

Technical characteristics

The typical medium composition:

Typical formulation:	g/ltr
Phytone peptone	10
Yeast extract	5
Sodium chloride	5
Agar	15

Fresh pH 7.0 ± 0.2

Applications

LB Agar is a medium used for general growth and maintenance of Escherichia coli strains. This medium is typically used in molecular biology procedures for cultivation of recombinant strains of E. coli for the detection of phage or plasmid transformed bacteria

Appearance

The prepared media is pale straw to straw in colour with a slightly opaque appearance.

Organisms & morphology

Microorganism	Observational appearance
Escherichia coli	Off-white colonies

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Typical incubation parameters

Incubation conditions can vary depending on the specific strain requirements. Typically, incubation range are within 35-37 °C for 18-24 hours.

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Growth Media Type Luria Broth Agar

Luria broth (LB) agar contains the generic components such as peptone, yeast extract, sodium chloride (NaCl) and agar which is useful to support microbial growth

Other Common Names

Primary Use

Technical characteristics

Applications

Appearance

Organisms & morphology

Typical incubation parameters

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