This product is widely known as nutrient agar.


Primary Use

Nutrient agar is a general-purpose medium used for the cultivation of non-fastidious microorganisms.

 

 

Technical characteristics


The typical medium composition:

Typical formulation: g/ltr
'Lab-Lemco' powder 1
Yeast extract 2
Peptone 5

Sodium chloride

5
Agar 15

Fresh pH 7.4 ±0.2

Applications


Nutrient Agar is a general purpose, non-selective nutritious media which allows maximum recovery of delicate microorganisms without affecting their haemolytic reactions. Media can be used to subculture microorganisms for maintenance purposes or to check the purity of subcultures from isolation plates prior to biochemical or serological tests.

Nutrient Agar contains a concentration of 1.5% agar to permit the addition of up to 10% blood or other biological fluid (such as serum or egg yolk), as required for cultivation of fastidious microorganisms. 

Related reading:


Appearance

The prepared media is pale straw to straw in colour with a slightly opaque appearance.

 

Microorganism

Observational appearance

Staphylococcus aureus

1-2 mm White / Straw colonies

Pseudomonas aeruginosa

1-3 mm Straw colonies

Yersinia enterocolitica

1-3 mm Straw colonies

Escherichia coli

1-3 mm Straw colonies

Salmonella enteritidis

1-3 mm Straw colonies

 

Typical incubation parameters

As nutrient agar is a general-purpose medium, it can be incubated at differing temperatures depending on the target microorganism. Typically, incubation range are within 37 ± 2 °C for 24 ± 2 hours.

 

 

Does Plate Choice Change Sampling Accuracy?

Comparative Analysis of Petri Dish Variation in Flow Rate for Air Sampling. This extended report aims to further investigate the variations in flow rate observed in petri dishes used for air sampling, focusing on the potential factors contributing to these fluctuations.


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